PBMC from normal, healthy donors were screened for NK activity using the CTL TVA™ Platform, and the percentage of killing is shown for K562 tumor cells. PBMC samples were incubated at different ratios of Effector Cells (PBMC containing NK cells) to Target Cells (K562 tumor cells labeled with fluorescent TVA™ Dye.) Following a four hour incubation period, the remaining labeled Target Cells were counted using the ImmunoSpot® TVA™ Platform, and the percentage of killing at each ratio was determined. The resulting killing curves are shown below.
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