CTL-Wash™ Supplement 10x is an enhanced serum-free cell culture medium specialized and optimized for individual washing steps. Our medium is based on injection-grade water (sterile- and endotoxin-free) and supplemented at ideal concentrations with amino acids, vitamins, inorganic salts and human serum albumin (HSA) for PBMC survival between working steps, where washing steps need to be included.
100 ml CTL-Wash™ Supplement 10x is ready to use after dilution in 900 ml sterile RPMI-1640.
CTL-Wash™ contains insulin, transferrin and other defined proteins, as well as saturated and unsaturated fatty acids and sugars in a proprietary composition to provide a balanced environment for maximizing PBMC survival.
Washing steps can or have to be included for example after density gradient isolation or after thawing. If cells are resting too long they can decrease their viability.
Avoid to add serum to CTL-Wash™. Serum can be contaminated with mitogens.
Available in 5 pack of 100ml bottles (Catalog #CTLW-010-5)