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Serum-free Media
Developed specifically for low background and high signal in cellular immune assays.
Outperforms the best sera in Immune Monitoring studies.

CTL-Test™ Medium
For direct ex vivo testing of PBMC in cellular cytokine assays (ELISPOT, ELISA, CBA, CPA)
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CTL-Test™ PLUS Medium
Facilitates detection of rare antigen-specific Th1, Th2, and Th17 memory cells
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CTL-Test™ B Medium
Formulated for expansion and ex vivo testing of PBMC in B cell ELISPOT and ELISA assays
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CTL-Wash™ Supplement 10x
Nutrient-rich supplement for PBMC to maintain full viability and functionality
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CTL-Cryo™ ABC Media Kit
Serum-free freezing media kit for cryopreservation of freshly-isolated PBMC
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CTL Anti-Aggregate Wash™ 20x Solution
Stabilizes PBMC during thawing and prevents cell loss due to aggregation
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Performance of Serum-free CTL Test™ Medium Compared with Eight Difference Qualified Sera
Cryopreserved PBMC of the same batch were thawed and tested in an IFN-γ ELISPOT assay in eight different laboratories using either CTL-Test™ (blue), or, in parallel in the serum that the respective laboratory has selected for T cell work (green). Spot counts obtained in the medium control are shown in Panel A. The antigen- (CEF peptide-) induced spot counts are shown in Panel B. The stimulation index (St: antigen-induced spots/medium background) defining the strength of signal measured is also shown for each of the laboratories for the results obtained with CTL-Test™ (SI: CTL- Test™), and with the respective laboratory's serum (SI: Serum). In all cases, the maximal spot counts with CTL-Test™ were equal to better than the sera, and four of the eight sera induced an elevated background (Zhang et al., J. immunotoxicology, 2009, 6:227).
