Our T-Cell reactivity characterized ePBMC® has been characterized for peptide and protein antigen reactivity on the frequency of the different T-Cell subpopulation (TH1, TH2, and TH17) which are secreting the signature cytokines IFN-γ, IL-2, IL-4, IL-5, and IL-17 in response to several MHC Class I- and MHC Class II-restricted antigens.
About T-Cell reactivity characterized ePBMC®
T-Cells are along with B-Cells the main component of the adaptive immune system.
T-Cells represent a heterogeneous group with different subspecies, performing various tasks. The different T-Cell subpopulations can easily be discriminated by their cytokine secretion profile and by a characteristic signature cytokine. The different cytokines determine the function of the T-Cell.
TH1-Lymphocytes are mediating the cellular immune response to an antigen. Via the signature cytokine IFN-γ - TH1-Cells enhance phagocytosis and the killing of internalized pathogens.
TH2-Lymphocytes mediating the humoral immune response to an antigen. Via the signature cytokine IL-4 -TH2-Cells induce a class-switch towards IgE class type in B-cells. In addition, the other signature cytokine IL-5 activates mature eosinophils.
TH17-Lymphocytes are similar to TH1-Lymphocytes, which are a part of the cellular immune response. The signature cytokine is IL-17 is the one which recruits neutrophils to the side of infection.
| ≥10 million cells per vial | Cat# CTL-IP1 |
|---|
Protocols
- Product Data Sheet Characterized Cryopreserved Human PBMC
- Thawing Cryopreserved PBMC
- Isolation of PBMC from Fresh Whole Blood
- Cryopreservation of PBMC from Fresh Whole Blood
- Plating PBMC into the ELISPOT Assay
Literature
- Resting of Cryopreserved PBMC Does Not Generally Benefit the Performance of Antigen-Specific T Cell ELISPOT Assays
- Optimal Thawing of Cryopreserved Peripheral Blood Mononuclear Cells for Use in High-Throughput Human Immune Monitoring Studies
- Brochure Cryopreserved Human PBMC
- Brochure Serum-free Media