Our HLA-typed ePBMC® has been characterized for low- and high resolution. HLA-typing has been performed for MHC Class I and Class II allele variations.
The extensive PBMC library provides you with standard donor information, containing age/ethnicity/gender/blood type. Moreover, the HLA typing of the respective donor is being provided.
About characterized PBMC
The HLA-genes, which are all located at chromosome 6, contain the information of the different subunits of the Major Histocompatibility Complex (MHC) and are hence essential for the function of the immune system. The three genes HLA-A, HLA-B and HLA-C are subunits of the MHC-I, whereas HLA-DR, HLA-DQ and HLA-DP genes are forming subunits of the MHC-II. Different allele variations in any of these genes can have an impact on the immune function of the cell. The HLA typing itself is essential, if it for instance comes to an organ transplantation.
| ≥10 million cells per vial | Cat# CTL-HP1 |
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Please contact us if you are interested in the immune cell function of donors with a specific HLA allele type.
Applications
- ELISPOT Assays
- Cytokine ELISA Assays
- Flow Cytometry
- Cytokine mRNA Determinations (RT-PCR)
- Antibody Dependent Cell-mediate Cytotoxicity (ADCC)
- Intracytoplasmic Cytokine Staining (ICS)
- Cytokine Bead Arrays (CBA)
- Cytokine Protein Arrays (CPA)
- Tetramers/Pentamers
- Transmigration Assays
- Any applications that require live, functional PBMC
Protocols
- Product Data Sheet Characterized Cryopreserved Human PBMC
- Thawing Cryopreserved PBMC
- Isolation of PBMC from Fresh Whole Blood
- Cryopreservation of PBMC from Fresh Whole Blood
- Plating PBMC into the ELISPOT Assay
Literature
- Resting of Cryopreserved PBMC Does Not Generally Benefit the Performance of Antigen-Specific T Cell ELISPOT Assays
- Optimal Thawing of Cryopreserved Peripheral Blood Mononuclear Cells for Use in High-Throughput Human Immune Monitoring Studies
- Brochure Cryopreserved Human PBMC
- Brochure Serum-free Media