Choose your ePBMC® corresponding to your experiment

  • Uncharacterized PBMC

    Our uncharacterized ePBMC® provides reproductive results for most standardized laboratory methods. We can offer up to 1000 vials per donor which are consistent in viability, recovery and functionality. Enabling you to perform several experiments.

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Cells which have been tested for specific application

  • Human Leucocyte Antigen (HLA) genes can differ between donors. Our ePBMC® has been characterized for high- and low-resolution HLA-typing.

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  • Natural Killer cells (NK cells) are a subpopulation of PBMC and fulfil an important function in tumor suppression as well as killing virus infected cells. Our ePBMC® has been investigated for their NK activity regarding the percentage killing efficiency in multiple effector cells.

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  • T-Cells fulfil important functions in the adaptive immune system. Here, we can offer our customers ePBMC® which has been characterized for reactivity of CD4+ and CD8+ restricted antigens regarding their secretion properties of typical cytokines like IFN- γ, IL-2, IL-4, IL-5, and IL-17.

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  • Antibody-Dependent Cell-mediated Cytotoxicity (ADCC) reflects the killing mechanism of antibodies through effector cells. The efficiency of this process depends on the FcγR receptor. Polymorphisms in these genes can change their activity. Here we are offering our customers ePBMC® with characterized FcγRIIIA loci, which can also be used as positive control for your assay.

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  • Cytokines like interleukins (IL) are important for the communication between cells. Our ePBMC® samples are characterized for reactivity to IFN-γ, IL-2, IL-4, 1L-5, and IL-17. Control samples and control antigens can be used as a positive control for your specific experimental setup.

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Applications

Our ePBMC® are ideal for any kind of assay or experiment which investigates immune cell function.

ELISPOT Cytokine Protein Arrays (CPA)
Natural Killer (NK) cell activity Assays Cytokine mRNA Determinations (qPCR)
Flowcytometry Antibody-dependent cell-mediated cytotoxicity (ADCC)
Western Blot Transmigration Assay
Cytokine ELISA Assays Cytotoxicity Assays
Cytokine Bead Arrays (CBA) Toxicology Studies
Intracytoplasmic Cytokine Staining (ICS) Tetramers/Pentamers

Characterization

Our cells are regularly tested for their immunological properties to guarantee a high-quality standards for all of our vials and batches.

On the cytokine level, batches of vials are routinely tested for their secretion properties of certain key cytokines named IFN-γ, IL-2, IL-4, IL-5, and IL-17 with the ELISPOT assay.

To validate functional T-Cells, several ELISPOT assays are performed with CEF peptides from human Cytomegalovirus (CMV), Epstein-Barr Virus (EBV) and Influenza Virus. To investigate cytotoxic CD8+ T-Cell function, vials have been tested for reactivity to 32 Class-I individual CEF peptides and two Class-I CEF peptide pools. To review CD4+ T helper cell function, reactivity to 24 Class-II individual CEF peptides, one Class-II CEF peptide pool, and six Protein antigens (from Mosquito, Dust Mite, Mumps, Candida, PPD, PHA) have been tested. Additionally, cells have been tested for reactivity to the CPI-pool, a complete new developed peptide pool specifically for CD4+ T helper cells.

If you are additionally interested in a specific HLA type, our donors have been tested on high resolution HLA-typed for three Class I loci (A, B, C) and four Class II loci (DRB1, DQA1, DQB1, and DP). Moreover, we offer our customers fully characterized demographics, vaccination status and antigen reactivity corresponding to our donors. This is helpful in order to have a reference sample and designing designing further experiments.

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Convenience

Our ePBMC® vials are isolated from leukocytes collected by leukapharesis and are frozen freshly in CTL-Cryo™ ABC serum-free freezing medium.

The leukapharesis samples are collected in full compliance with the US HIPAA guidelines to eliminate any potential breach of privacy. To ensure that our samples are not contaminated with an infectious virus, we always serological tests of HBV surface antigen (HBsAg), Hepatitis B core antibody (HBcAb), Hepatitis C virus (HCV), Human T-Cell lymphotropic virus type 1 and type 2 (HTLV I/II) and sexual transmitted diseases (STD). Further, we used nucleid acid testing to detect a potential contamination with human immunodeficiency virus 1 (HIV I), HCV and West Nile virus (WNV).

We guarantee that our ePBMC® does not need any ethic proposal for working in your laboratory. We take over all regulatory approvals which accelerates your experimental setup.

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Instant Availability

Shipments to Europe take approximately 3 workdays. Our cells are shipped in Nitrogen vapor at -180C to ensure that our cells show the same functionality everywhere.