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Cryopreserved Human PBMC
Uncharacterized
Uncharacterized PBMC can be reserved in large of up to 300 - 400 vials for repeated use in assay development, as reference samples. Consistent viability, recovery and functionality.
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HLA-Type
Characterized for high- and low-resolution HLA-typing. Search our database to reserve batches of specific HLA types to match your needs.
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NK-Activity
PBMC have been characterized for direct NK killing activity against tumor cells. Percent killing efficiency at various effector to target ratios have been determined.
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T Cell Reactivity
PBMC characterized for reactivity to a variety of CD4+ and CD8+ antigens. IFN-, IL-2, IL-4, IL-5, and IL-17 recall responses have been determined for several Class I- and Class II-restricted antigens.
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ADCC Fc Receptor Polymorphism
Select cells from donors who have been characterized for polymorphisms at the FcγR3A loci. Polymorphisms in the FcγR3A gene have been shown to affect performance in ADCC PBMC can be used as assay controls or reference samples for ADCC.
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Cytokine Characterized Control Sample with Antigen
Samples are characterized for reactivity to IFN-γ, IL-2, IL-4, 1L-5, and IL-17. Control samples and antigen are provided for use as internal controls in clinical trials, or for assay development and validation.
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Applications
Individual healthy donor cells are available in batches of up to 1000 vials for any application that requires live, functional PBMC.
| ELISPOT | Cytokine Protein Arrays (CPA) |
| NK assays | Cytokine mRNA Determinations (RT-PCR) |
| Tetramers/Pentamers | ADCC |
| Intracytoplasmic Cytokine Staining (ICS) | Transmigration Assay |
| Cytokine ELISA Assays | Cytotoxicity Assays |
| Cytokine Bead Arrays (CBA) | Toxicology studies |
Characterization
Immune characterized PBMC are available in large batches of individual cryopreserved vials with predetermined frequencies of antigen-specific IFN-g, IL-2, IL-4, IL-5, IL-17 secreting cells.
Each batch of PBMC has been tested for reactivity in ELISPOT assays for Class I-restricted antigens: 32 Class I individual CEF-peptides and 2 Class I CEF-Peptide Pools and Class II antigens: 24 Class II individual CEF peptides, 1 Class II CEF-Peptide Pool, and 6 Protein antigens (Mosquito, Dust Mite, Mumps, Candida, PPD, PHA), as well as our new CPI antigen peptide pool, which was developed as a CD4+ specific positive control.
High resolution HLA-typed for three Class I loci (A, B, C) and four Class II loci (DRB1, DQA1, DQB1, and DP)
Fully characterized donor demographics, vaccination status and antigen reactivity.
Reference samples for designing your own experiments or for screening the reactivity of uncharacterized PBMC against your specific antigen or compound of interest.
Convenience
ePBMC® samples are isolated from leukocytes collected by leukapharesis and are frozen in CTL-Cryo™ ABC serum-free freezing medium.
The leukapharesis samples are collected in full compliance with HIPAA guidelines to eliminate any potential breach of privacy. They are tested for: HBsAg, HBcAb, HCV, HTLV I/II and STS by serology; as well as HIV I, HCV and WNV by NAT (nucleic acid testing).
No IRBs required – We’ve taken care of all ethical and regulatory compliance issues so that you can purchase donor samples matching your requirements with no hassle.
Additional Resources
- ePBMC® Brochure
- CTL Serum-free Media Brochure
- Protocols & Guidelines for Working with PBMC
- Resting of cryopreserved PBMC does not generally benefit the performance of antigen-specific T cell ELISPOT Assays
- Optimal thawing of cryopreserved PBMC for use in high-throughput human immune monitoring studies
- ELISPOT assays in 384-well format allow analysis of 400 independent T cell tests with 10ml blood
- ELISPOT assays provide reproducible results among different laboratories for T cell immune monitoring even in the hands of inexperienced investigators
