See why we believe in ePBMC!
Access to an extensiv donor library
Being an official distributor of Cellular Technology Limited, based in Cleveland, Ohio, we are having access to an extensive library of cryopreserved peripheral blood mononuclear cells, ePBMC®. We are able to offer our costumers instant access to characterized, standardized, and quality-controlled PBMC in virtually unlimited quantities.Search our Database here!
More Cost Effective than DIY PBMC
The actual cost for generating PBMC for your assays goes well beyond the cost for blood. If you were to characterize your PBMC, the effort would be substantial. Our ePBMC® are quality controled and come pre-characterized with established functionality. It takes less than 20 minutes to thaw and prepare ePBMC® for your assay.
Each donor is pre-tested for NK cytolytic activity and for antigen-specific T cell cytokine responses by measuring the secretion of IFN-γ, IL-2, IL-4, IL-5, and IL-17 in ELISPOT functionality testing.
Our mission is to provide our costumers the highest level of service. That is how we can guarantee processing their orders as smooth and efficient as possible. Our product specialists are glad to consult costumers with product related questions.
We are an official European distributor of Cellular Technology Limited's ePBMC® and Serum-free Media product lines. Contact us and join a new era of human research!
Technical Tips for working with Human Peripheral Blood Mononuclear Cell (PBMC) in ELISPOT Assays
- It is important to quick-spin vials before use to ensure content volumes.
- To maximize the use of each plate, an adhesive plate-sealing sheet has been included that can be adhered to the top of the plate to cover and protect unused wells that are intended to be used in subsequent assays. Use your thumbs to firmly adhere the sheet to the plate and use a razor blade to cut the sheet to expose only the necessary wells.
- We highly recommend the use of CTL Serum-free Media for freezing, washing, and testing PBMC. Even brief exposure to a mitogenic serum can cause high background while other sera can have suppressive effects. CTL also recommends using the CTL-LDCTM Kit for accurate live/dead cell counts.
- Deviations from specified temperatures, timing requirements, number of washing steps, and specified reagent preparation volumes may alter the performance of the assay.
- Plates can be washed manually or by a suitable automated plate washer with adjusted pin length and flow rate so membranes and spots are not damaged (CTL recommends BioTek ELx405).
- To avoid damage to the PVDF membrane in the wells, do not touch the membrane with pipette tips or with the plate washer. The PVDF membrane is permeable and protected by an underdrain. Avoid direct contact between the well bottom and wet surfaces, including paper towels or any other materials that can absorb liquid.
- While processing plates, the PVDF membrane at the bottom of the wells must remain wet.
- When underdrain and gloves are wet, the underdrain may be slippery and difficult to remove. Wipe gloves and underdrain with paper towel before removing.
- After completion of the experiment, do not dry the ELISPOT assay plates at temperatures exceeding 37°C as this may cause the membrane to crack.
- Spots may not be readily visible while the membrane is still wet. Scan and count plates only after membranes have completely dried.
- Higher background appearing in the control wells can be potentially overcome by the following steps: – When working with pre-cultured cells, wash the cells thoroughly in CTL-WashTM prior to the experiment in order to avoid carryover of cytokines and other substances; use CTL-TestTM for testing PBMC.