CTL Anti-Aggregate Wash™ Supplement 20x solution contains injection-grade water supplemented with amino acids, vitamins, inorganic salts, and human serum albumin in a proprietary composition to provide a nutritionally complete and balanced environment for the PBMC. It contains DNAse that inhibits cell clumping after thawing the PBMC.
Stabilizes PBMC during thawing and prevents cell loss through aggregation. Cells that die during the cryopreservation and thawing processes release DNA strands that can cause cell clumping and aggregation. Therefore, the use of DNAse-containing wash solutions is recommended while thawing cryopreserved PBMC. PBMC show increased metabolic activity following thawing as they compensate for the stress reaction — their recovery can be accommodated by using a nutrient-rich medium.
CTL Anti-Aggregate Wash™ Supplement has been specifically formulated for optimal PBMC recovery and functionality after thawing cells that have been cryopreserved.
Anti-Aggregate Wash Supplement contains DNAse, insulin, transferrin, and human albumin, among other defined proteins in a proprietary composition, saturated and unsaturated fatty acids, and sugars in a ready-to-use formulation, providing a balanced environment for PBMC.
For each vial of PBMC to be thawed, 1 vial of CTL Anti-Aggregate Wash™ Supplement 20x is needed.
Available in 1ml (sufficient to prepare 20ml; Catalog #CTL-AA-001), and 5ml (sufficient to prepare 100ml; Catalog #CTL-AA-005)